Streptococcus pneumoniae, a Gram-positive obligate human pathogen that colonizes mucosal surfaces in
the host can cause a broad range of severe diseases like pneumonia, septicaemia, otitis media, sinusitis and meningitis. The high number of approximately 12.000 deaths per year in Germany (RKI)
and 1.6 mio worldwide (WHO) due to invasive infections with this pathogens displays the need for knowledge regarding pneumococcal infections .
Since the knowledge about the pneumococcal metabolism is very limited, we aimed
for a metabolomics approach that would allow us to investigate basic metabolic characteristics during growth in a chemical defined medium. By doing so we used different growth conditions for two
S. pneumoniae strains and analyzed metabolic footprints and fingerprinting using 1H-NMR, GC-MS or HPLC-MS. Also we are interesting on antibiotic profile.The project is fundedby the DFGandinvolvedin theRTG1870.
 (2013) Progress in introduction of pneumococcal conjugate vaccine - worldwide,
2000-2012. MMWR. Morbidity and mortality weekly report 62 (16): 308–311.
Metabolome analysis of Gram-positive Bacteria, such as Staphylococcus aureus and Bacillus subtilis under growth-limiting
Analysis of the nutrients availability in different human body fluids that reflect natural habitats of Staphylococcus aureus during host