Research of Pathogens interaction

Streptococcus pneumoniae

Anne Leonard

 

Streptococcus pneumoniae, a Gram-positive obligate human pathogen that colonizes mucosal surfaces in the host can cause a broad range of severe diseases like pneumonia, septicaemia, otitis media, sinusitis and meningitis. The high number of approximately 12.000 deaths per year in Germany (RKI) and 1.6 mio worldwide (WHO) due to invasive infections with this pathogens displays the need for knowledge regarding pneumococcal infections [1].

Since the knowledge about the pneumococcal metabolism is very limited, we aimed for a metabolomics approach that would allow us to investigate basic metabolic characteristics during growth in a chemical defined medium. By doing so we used different growth conditions for two S. pneumoniae strains and analyzed metabolic footprints and fingerprinting using 1H-NMR, GC-MS or HPLC-MS. Also we are interesting on antibiotic profile. The project is funded by the DFG and involved in the RTG 1870.

 

[1] (2013) Progress in introduction of pneumococcal conjugate vaccine - worldwide, 2000-2012. MMWR. Morbidity and mortality weekly report 62 (16): 308–311.

Staphylococcus aureus

Kirsten Dörries

 

Metabolome analysis of Gram-positive Bacteria, such as Staphylococcus aureus and Bacillus subtilis under growth-limiting conditions.

Analysis of the nutrients availability in different human body fluids that reflect natural habitats of Staphylococcus aureus during host colonization.